Abstract
1. 1. [ 14C]Chloramphenicol is taken up by intact Bacillus megaterium when incubated in a suitable growth medium. In a cell-free system containing either K + or NH + 4, the antibiotic is bound specifically to the 50-S subunit of the ribosomes. 2. The uptake of [ 14C]chloramphenicol by B. megaterium and its binding to bacterial ribosomes is prevented by d-thiocymetin, d-win 5094, streptogramin, streptogramin A, lincomycin, celesticetin and all seven macrolide antibiotics tested. Streptogramin B, staphylomycin S and viridogrisein reduce the uptake of [ 14C]-chloramphenicol by intact bacteria but have no effect on the binding of [ 14C]chloramphenicol to ribosomes in cell-free systems. 3. The effects of some of the compounds on the ribosome are reversible whereas other antibiotics appear to affect the ribosome irreversibly.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
