Abstract
Phosphorus-31 NMR lines corresponding to inner and outer surfaces of sonicated phosphatidylserine vesicles can be distinguished by the effects of added Ca 2+ or Mg 2+ at low bulk concentrations (millimolar or less). The changes in chemical shift and relaxation times indicate that Ca 2+ binds directly to the PS phosphate, neutralizing at least a portion of the negative charge and restricting the motion of this group. Mg 2+ ion also binds to the head group, but apparently not as strongly as Ca 2+, nor is the mobility of the headgroup affected as much.
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