Abstract
Reactions between radiolabeled heat-denatured E. coli DNA and antibodies specific for each of the four naturally occurring mononucleosides were detected by the modification of the Farr procedure using antigamma globulin to precipitate the antigen-antibody complex. Nonimmune serum binds less than 5 per cent of the radiolabeled DNA. The homologous hapten is at least 10 3 times more inhibitory than the heterologous haptens. Formaldehyde-denatured DNAs are also effective inhibitors. M. lysodeikticus DNA‡ (72 per cent G-C), denatured in the presence of 1% formaldehyde inhibited anti-cytidine better than did Cl. perfringens DNA (30 per cent G-C) similarly denatured. The reverse was obtained with antithymidine. Thus DNAs of varying base composition can be differentiated from each other.
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