Abstract

The hydroxyl form of a strongly basic ion-exchange resin, Dowex-1 X8, had been used by Yamaguchi et al. to separate alditols from monosaccharides for estimation of the degree of polymerization (d.p.) of neutral oligosaccharides and polysaccharides, because alditols passed through a column of the resin, but monosaccharides were bound to it. However, the resin has now been found to adsorb not only monosaccharides but also alditols, and it is difficult to separate them readily. Therefore, this method for estimation of the d.p. of oligosaccharides has been improved. Elution of alditols from a column of Dowex-l X8 ( OH -) resin is influenced by methanol, ammonium acetate, and ammonium formate.

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