Binding Capacity for Aflatoxin B 1 by Different Adsorbents

Abstract

In this article, in vitro adsorption of aflatoxin B 1 (AFB 1) onto different adsorbents was characterized and the result was verified by comparing the growth performance and serum protein levels of broilers exposed to aflatoxin-contamination feed. Main components of adsorbents selected were yeast cell extracts (Product A), HSCAS (Product B), and a mixture of yeast product and HSCAS (Product C), respectively. A total of 240 broilers were assigned to eight treatments, and the effects of three types of adsorbents on growth performance and serum protein levels were evaluated. Results indicated that Product B had the highest in vitro affinity for AFB 1, followed by Product C and Product A. Product B bound 97.69% of the AFB1 in solution in 10 min, and it remained over 96.03% in 60 min at pH 8.0. The B-AFB 1 complex was much stronger than the other two complexes in vitro condition ( P < 0.05). Feed intake (FI) and average daily gain (ADG) decreased ( P< 0.05) and feed gain ratio increased ( P < 0.05) in the treatment fed aflatoxin-contaminated feed versus treatment on the basal feed. Serum total protein (TP), albumin (ALB), and globulin (GLOB) levels were significantly decreased ( P< 0.05). Product B (0.15%) increased growth performance and improved serum protein levels, Product A and Product C were not as effective as Product B. Three adsorbents tested here had sufficient potential to AFB 1 in some extents and Product B could bind AFB 1 more effectively than Product A and Product C. These results indicated that Product B could alleviate some of the AFB 1 toxic effect in broilers.