Binding and release of ouabain at low Mg2+ in reconstituted human red blood cell ghosts

Abstract

Various conformational states of Na,K-ATPase as pharmacological receptor for cardiac glycosides are recognized by the rate of ouabain binding and release in porous and reconstituted human red blood cell ghosts. At low Mg2+, conformational changes become evident in the presence of various ligands which cannot be studied at physiological concentrations of Mg2+. By using reconstituted human red cell ghosts containing ATP and little Mg2+, it is possible to evaluate the side-specificity of those effects of Na+ and K+ which have been observed in porous ghosts. Some of the following data are detailed in previous papers (5, 6).KeywordsPhysiological ConcentrationConformational StateCardiac GlycosideCholine ChlorideBinding RateThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.