Abstract
The bimolecular fluorescence complementation (BiFC) assay is a powerful, flexible, and simple tool to study protein-protein interactions in living cells. To accelerate the production and assessment of BiFC constructs, Gateway-compatible multicolor BiFC vectors were generated to enable the simultaneous production of multiple fusion genes that have the split N- or C-terminal fragment of fluorescent protein with the gene of interest in a high-throughput manner. Two different transient expression techniques for the assessment of BiFC in plant cells are described.
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