Abstract
Cell migration is one of the key cell functions in physiological and pathological processes, especially in tumor metastasis. However, it is not feasible to monitor the important biochemical molecules produced during cell migrations in situ by conventional cell migration assays. Herein, for the first time a device containing both electrochemical sensing and trans-well cell migration modules was fabricated to sensitively quantify biochemical molecules released from the cell migration process in situ. The fully assembled device with a multi-wall carbon nanotube/graphene/MnO2 nanocomposite functionalized electrode was able to successfully characterize hydrogen peroxide (H2O2) production from melanoma A375 cells, larynx carcinoma HEp-2 cells and liver cancer Hep G2 under serum established chemotaxis. The maximum concentration of H2O2 produced from A375, HEp-2 and Hep G2 in chemotaxis was 130±1.3 nM, 70±0.7 nM and 63±0.7 nM, respectively. While the time required reaching the summit of H2O2 production was 3.0, 4.0 and 1.5 h for A375, HEp-2 and Hep G2, respectively. By staining the polycarbonate micropore membrane disassembled from the device, we found that the average migration rate of the A375, HEp-2 and Hep G2 cells were 98±6%, 38±4% and 32 ±3%, respectively. The novel bi-module cell migration platform enables in situ investigation of cell secretion and cell function simultaneously, highlighting its potential for characterizing cell motility through monitoring H2O2 production on rare samples and for identifying underlying mechanisms of cell migration.
Highlights
Cell migration plays a role in many physiological and pathological processes, including tumor metastasis.[1,2,3] It is a physical and chemical multistep cycle including extension of a protrusion, formation of stable attachments near the leading edge of the protrusion, translocation of the cell body forward, and release of adhesions and retraction at the cell rear.[4,5,6] CellPLOS ONE | DOI:10.1371/journal.pone.0127610 June 2, 2015In Situ Sensing Hydrogen Peroxide Release from Migrating Cells
Our previous study demonstrated that multi-wall carbon nanotube (MWCNT)/graphene/MnO2 responses to H2O2.[41]. To evaluate the stability of the sensor that immersed in cell culture medium for 24 h, 1 mM H2O2 was added into the medium at 0, 12, 18 and 24 h, and the cyclic voltammetry (CV) response was recorded
To realize H2O2 production in situ, attention has been paid on the amperometric response of the MWCNT/graphene/MnO2 functionalized device to subsequent additions of H2O2 in cell culture medium (RPMI 1640)
Summary
Cell migration plays a role in many physiological and pathological processes, including tumor metastasis.[1,2,3] It is a physical and chemical multistep cycle including extension of a protrusion, formation of stable attachments near the leading edge of the protrusion, translocation of the cell body forward, and release of adhesions and retraction at the cell rear.[4,5,6] CellPLOS ONE | DOI:10.1371/journal.pone.0127610 June 2, 2015In Situ Sensing Hydrogen Peroxide Release from Migrating Cells. Cell migration plays a role in many physiological and pathological processes, including tumor metastasis.[1,2,3] It is a physical and chemical multistep cycle including extension of a protrusion, formation of stable attachments near the leading edge of the protrusion, translocation of the cell body forward, and release of adhesions and retraction at the cell rear.[4,5,6] Cell. In Situ Sensing Hydrogen Peroxide Release from Migrating Cells
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
