Abstract
This study aims at investigating the efficiency of bimodal spectroscopy in detection of hypertrophic scar tissue on a preclinical model. Fluorescence and Diffuse Reflectance spectra were collected from 55 scars deliberately created on ears of 20 rabbits, amongst which some received tacrolimus injection to provide non-hypertrophic scar tissue. The spectroscopic data measured on hypertrophic and non-hypertrophic scar tissues were used for developing our classification algorithm. Spectral features were extracted from corrected data and analyzed to classify the scar tissues into hypertrophic or non-hypertrophic. The Algorithm was developed using k-NN classifier and validated by comparing to histological classification result with Leave-One-Out cross validation. Bimodal spectroscopy showed promising results in detecting hypertrophic tissue (sensibility 90.5%, specificity 94.4%). The features used for classification were extracted from the autofluorescence spectra collected at 4 CEFS with excitations at 360, 410, and 420 nm. This indicates the hypertrophic process may involve change in concentration of several fluorophores (collagen, elastin and NADH) excited in this range, or modification in volume of explored tissue layers (epidermis and dermis) due to tissue thickening.
Highlights
Hypertrophy is defined as an increase in tissular volume of an organ, resulting from normal physiological process [1] or caused by abnormal accumulation of tissular components, such as hypertrophic scars or keloids occurred on human skin
The 4 selected slopes are those of AF intensity spectra collected at excitation wavelengths 360, 410 and 420 nm, which puts forward their specific discrimination efficiency and the relationship between hypertrophic tissues and the constitutive fluorophores excited at these wavelengths
The efficiency of bimodal spectroscopy was investigated in the detection of hypertrophic scar tissue on a preclinical model
Summary
Hypertrophy is defined as an increase in tissular volume of an organ, resulting from normal physiological process [1] or caused by abnormal accumulation of tissular components, such as hypertrophic scars or keloids occurred on human skin. Kischer and Brody [3] identified collagen nodules to be the structural unit of hypertrophic scars and keloids These nodules, which are absent from mature scars, contain higher density of fibroblasts and unidirectional collagen fibrils in a highly organized and distinct orientation. The dermal lymphocytes and fibroblasts densities were examined for evaluating the cellular proliferation of underline tissue Based on these measurements, two quantitative parameters were calculated to characterize the tissue samples : Dermal Fibroblasts Density (DFD) and Scar Elevation Index (SEI) [23].
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