Bimodal Gigaporous Polystyrene Microspheres with Glycopolymer Surfaces for High-Speed Protein Chromatography

Abstract

A bimodal gigaporous anion-exchange medium was successfully developed based on hydrophilic gigaporous polystyrene (HGPS) microspheres that prepared previously. After derivatization, the gigaporous structure of HGPS microspheres was well maintained. The presence of flow-through pores in the medium can induce convective flow of mobile phase within microspheres, therefore contributing to high column permeability (3.55×10-10 m2), high dynamic adsorption capacity and high protein resolution at high flow velocities. The mixture of hemoglobin, transferrin and ovalbumin can be baseline separated within 3 minutes at 2529 cm/h. All the results indicate that anion-exchange medium as developed is very promising for large scale high-speed protein chromatography.