Abstract

We replace a commonly used Au film with an Au/Ag bimetallic film working as the sensing chip in the commercial surface plasmon resonance (SPR) instrument, Biacore 3000. The bimetallic chip gives out sharper SPR dip than that by the chip made of an Au film which can improve the sensitivity and accuracy. The applicability and stability of the bimetallic chip on the Biacore 3000 are tested by real-time monitoring of biological coupling processes between Sulfamethoxazole (SMX) and anti-SMX. The limit of detection of SMX is 1.8ng/ml.

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