Bilirubin Removal from Human Plasma with Albumin Immobilised Magnetic Poly(2‐hydroxyethyl methacrylate) Beads

Abstract

AbstractBioaffinity separation has a unique and powerful role as a support tool in the removal of toxic substances from human plasma. Magnetic beads have advantages as supports in comparison to conventional nonmagnetic beads because of low pressure drop, high mass transfer rates, and good fluid‐solid contact. In addition, they eliminate internal diffusion limitations. Human serum albumin (HSA) immobilised onto magnetic poly(2‐hydroxyethyl methacrylate) (mPHEMA) beads were investigated as an adsorbent for the selective bilirubin removal from human plasma. The mPHEMA beads were prepared by a modified suspension polymerisation. HSA was covalently coupled to the mPHEMA beads. Bilirubin adsorption was investigated from hyperbilirubinemic human plasma on the mPHEMA beads containing different amounts of immobilised HSA, (between 11–100 mg/g). The nonspecific bilirubin adsorption on the unmodified mPHEMA beads was 0.47 mg/g. Higher bilirubin adsorption capacities, up to 64.7 mg/g, were obtained with the HSA‐immobilised magnetic beads. Bilirubin adsorption increased with increasing temperature.Effect of HSA loading on bilirubin adsorption.magnified imageEffect of HSA loading on bilirubin adsorption.