Abstract
Investigations of the influence of unconjugated bilirubin and free hemoglobin on the ceric oxidation BEI method of Posner have revealed the following: 1. (a) Addition of bilirubin or free hemoglobin to standard sera or to butanol extracts of standard sera significantly reduces serum hormonal iodine recoveries. 2. (b) This effect is not related to inhibition of the direct ceric oxidation of thyroxine iodine. 3. (c) Bilirubin added to the final ceric-arsenious mixture reacts with ceric ion so that the resultant product is colorless and nonreactive to benzaldehyde or diazo reagents. The colorless product also inhibits the ceric-arsenious reaction. The structure of the reaction product(s) is not known. 4. (d) Pre-extraction of serum with chloroform leads to inhibition by chloroform of the final reaction. Moreover, chloroform extraction, as conducted, was incomplete and results using pre-extraction of sera containing in excess of 3 mg 100 ml bilirubin were unreliable.
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