Bile Salts against Ehrlich Ascites Carcinoma Cells

Abstract

Surfactants, which are generally good lytic agents for biological membrane have a brilliant possibility to be useful either as a drug or as agent which may help transport of another drug across the cell membrane by changing membrane fluidity. Here we have studied the whole cell lysis with bile salts using Enrlich Ascites Carcinoma (EAC) cells. This investigation is important since bile salts should be less toxic to the normal organ than synthetic detergents. Na-cheno and Na-deoxycholate are more effective lytic agents than Na-cholate for EAC cell membrane lysis while Na-dehydrocholate did not cause any lysis of EAC cells. Specific role of hydroxyl group in the bile salts is apparent EAC cell membrane is more sensitive to the bile salts during the stationary phase of growth than the log phase. Studies under polarizing microscope show that cell lysis is time dependent Preliminary in vivo study indicates that chenodeoxycholate is toxic to the host. Na-cholate treatment with 45 mg/kg shows increase of life span over the control. In vivo studies for the effect of treatment of Na-cholate on the blood of normal healthy male Swiss A mice indicated initial damage of the blood parameters which were found to be recovered with time and the dose was not found to be lethal to the animals.