Abstract
The use of 2-hydroxypropyl-beta-cyclodextrin has made it possible to prepare stable aqueous solutions of cholesterol, 26-hydroxycholesterol, 7 alpha-hydroxycholesterol, and monohydroxy bile acids such as lithocholic and 3 beta-hydroxy-5-cholenoic acids. These solutions are suitable for cell culture studies and for parenteral administration to animals.
Highlights
Summary The use of 2-hydroxypropyl-byclodextrin has made it possible to prepare stable aqueous solutions of cholesterol,26-hydroxycholesterol,7a-hydroxycholestero1,and monohydroxy bile acids such as lithocholic and 3fkhydroxy5-cholenoicacids.Thesesolutionsaresuitableforcell culture studies and pfoarenteardalministratiton animals.-De Caprio, J., J
Since the introduction of anexperimentalmodel for the rapid induction ofcholestasis by intravenous infusion of monohydroxy bile acids [1, 2], a major technical problem has beentopreparean infusion mixture that maintains these compounds in aqueous solution [3], and we ourselveshave tried numerous procedures without success
The tubes were centrifuged and analiquot of the clear solution was taken for analysis by GLC after formation of the methyl ester acetate for bile acids and the mono- or diacetates for sterols using methods for their preparation that were described in detail previously [4,5].Internal standards were 3a, 7adihydroxy-12-oxo-5bholanoic acid for bile acids and coprostanol forsterols
Summary
Summary The use of 2-hydroxypropyl-byclodextrin has made it possible to prepare stable aqueous solutions of cholesterol,26-hydroxycholesterol,7a-hydroxycholestero1,and monohydroxy bile acids such as lithocholic and 3fkhydroxy5-cholenoicacids.Thesesolutionsaresuitableforcell culture studies and pfoarenteardalministratiton animals.-De Caprio, J., J. Supplementary key words lithocholic acid 3P-hydroxy-5-cholenoic acid cholesterol 26hydroxycholesterol steroids bile acids Since the introduction of anexperimentalmodel for the rapid induction ofcholestasis by intravenous infusion of monohydroxy bile acids [1, 2], a major technical problem has beentopreparean infusion mixture that maintains these compounds in aqueous solution [3], and we ourselveshave tried numerous procedures without success.
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