Bi-layered Nanofibers Membrane Loaded with Titanium Oxide and Tetracycline as Controlled Drug Delivery System for Wound Dressing Applications.

Rezk Rezk,Lee Lee,Park Park,Son Son,Kim Kim

doi:10.3390/polym11101602

Abstract

The aim of this study is to develop a novel functional bi-layered membrane loaded titanium oxide (TiO2) and tetracycline (TTC) for application in wound dressing. The advantages of the electrospinning technique have to be considered for the uniform distribution of nanoparticles and TTC drug. The as prepared nanofibers and TiO2 were characterized in terms of morphology, fiber diameter, mechanical properties and surface wettability. The in vitro drug release study revealed initial burst release followed by a sustained control release of TTC for 4 days. The in vitro antibacterial of the bi-layered nanofibers was conducted against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria species showing excellent antibacterial effect for drug loaded samples compared with PCL nanofibers. Subsequently, cell counting kit-8 (CCK-8) and confocal laser scanning microscopy (CLSM) were used to evaluate its biocompatibility in vitro. Our results revealed that the bi-layered membrane has better antibacterial and cell compatibility than the control fiber. This suggests that the fabricated biocompatible scaffold is appropriate for a variety of wound dressing applications.

Highlights

Wound dressings are attracting substantial, widespread attention in chronic wound therapy, such as for diabetic chronic ulcers, which remains a significant clinical challenge due to the limited treatment routes and suboptimal healing outcomes
The first layer represents pure PCL to maintain the mechanical properties of the nanofiber mat, while the second layered is composed of PDO loaded with nanoparticles and TTC drug molecules to provide an antibacterial effect
We revealed that the mechanical strength of PP3T5T and PP5T5T nanofiber could fulfill the basic requirements for wound dressing application

Summary

Introduction

Wound dressings are attracting substantial, widespread attention in chronic wound therapy, such as for diabetic chronic ulcers, which remains a significant clinical challenge due to the limited treatment routes and suboptimal healing outcomes. An ideal wound dressing scaffold should have the same function and structurally similar to skin tissue in order to promote cell proliferation and adhesion, isolate the wound from adverse environments and avoid secondary damage to the wound, providing mechanical strength and accelerating the wound healing [2]. In order to mimic the assembly and function of skin, wound dressings with a bi-layered model have been introduced. The scaffolds provide a barrier against bacterial infection and water loss, and have a tridimensional structure with interconnected pores in wounds. These scaffolds still have some disadvantages, including cost-associated concerns, infection risks factors, and temporary coverage [4,5]

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