Abstract
A bilayer composite scaffold consisting of a biphasic calcium phosphate–pectin–gelatin hydrogel and an electrospun poly(vinyl alcohol)–gelatin matrix was developed and investigated for application in bone hemorrhage. The aim of this research is to develop a new biomaterial system that provides a hemostatic effect on bone hemorrhage and does not interfere with native bone regeneration. The role of electrospun poly(vinyl alcohol)–gelatin in scaffolds is to provide the covering of the wound, while encapsulation and absorption of red blood cells are attributed to the biphasic calcium phosphate–pectin–gelatin hydrogel. Cell viability and cell proliferation were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Live/Dead assay, and immunofluorescence assay using pre-osteoblast MC3T3-E1 cells. The effects with and without hemostatic bilayer scaffolds on rat calvaria were compared at 1 and 3 weeks. The hemostatic bilayer agent showed good blood absorption behavior and efficient bone healing properties after 3 weeks, as observed by micro-computed tomography and hematoxylin and eosin staining.
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