Bilaterally projecting neurons in the two visual pathways of chicks

Abstract

By means of a double-labeling technique, we have investigated the organization of the bilateral thalamo-Wulst and tecto-rotundal projections in 2-day old chicks. After injecting fluorogold (FG) into one side of the visual Wulst and rhodamine B isothiocyanate (RITC) into the other side of the visual Wulst, the labeled neurons in the nucleus geniculatus lateralis pars dorsalis (GLd) were examined. Although the distribution areas of ipsilaterally and contralaterally labeled neurons overlap partly, very few double-labeled neurons were found (only 0.01% double-labeled neurons). This suggests that the ipsilateral and contralateral projections to the Wulst come from different neuronal populations of the thalamus. The FG and RITC were also injected into the rotundal nuclei (Rt) on each side of the thalamus and the labeled neurons in the optic tectum (TeO) were examined. In the TeO, the distribution areas of the neurons labeled ipsilaterally and contralaterally to Rt overlap completely and we found that up to 45% of the tectal cells were double-labeled by both FG and RITC. Therefore, many tectal neurons have axon collaterals so that they project to the Rt on both sides of the thalamus and must send information simultaneously to both sides of the brain. The differences in the structural organization of the two visual pathways are discussed with reference to the transmission of information to higher centers on both sides of the brain.