Bilateral anterior elevation prosthesis boosts chondrocytes proliferation in mice mandibular condyle.

Abstract

We aimed to develop a mouse model predominating in a proliferative response in the articular cartilage of the temporomandibular joints. Bilateral anterior elevation of occlusion was developed by installing metal tubes onto the incisors of mice with edge-to-edge relation to prevent tooth wear, leading to an increase in the vertical height of the dental occlusion with time. Morphological changes and expression changes in Cyclin D1, Aggrecan, and type II and type X collagen in the mandibular condylar cartilage were detected. In addition, cells were isolated from the mandibular condylar cartilage and exposed to cyclic tensile strain (CTS). Compared with age-matched controls, the tooth length was longer at 3weeks, 7weeks, and 11weeks in BAE mice (p<0.05), with increased condylar cartilage thickness, matrix amount, and cell number (p<0.05). Compared with the deep zone cells, CTS stimulated the superficial zone cells to express a higher level of proliferating cell nuclear antigen, Cyclin D1, Aggrecan, and type II collagen but a lower level of type X collagen and alkaline phosphatase. Bilateral anterior elevation stimulated the proliferative response in the mandibular condylar cartilage, offering a new therapeutic strategy for cartilage degeneration.