Abstract
SummaryFreeze-fracturing and freeze-etching have provided a great deal of details about intra- and extramembranous particles corresponding to intrinsic and extrinsic thylakoidal proteins, and about their distribution, clearly demonstrating the asymetrical nature of the thylakoidal membrane and its fluidity. Some groups of particles have been identified, but the exact nature of the majority remains to be clearly established. Direct methods (selective extraction or enzymatic digestion of proteins) or indirect methods (study of photosynthetic mutants for instance) coupled to freeze-etching and freeze-fracturing studies will permit to go thoroughly into knowledge about photosynthetic apparatus.
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