Abstract
Using a radioimmunoassay for γ 3-melanotropin ( γ 3-MSH), γ-melanotropin-like immunoreactivity (γ-MSH-LI) was detected in plasma extracts of normal subjects before and after metyrapone administration. Plasma γ-MSH-LI from four normal men rose significantly after a single oral administration of metyrapone (30 mg/kg of body weight). Gel chromatographic study of plasma extract after metyrapone administration showed a single peak of γ-MSH-LI near the elution position of mouse 16K fragment, however smaller forms of γ-MSH-LI were not detected.
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