Abstract
Mesenteric ischemia/reperfusion is a clinical emergency with high morbidity and mortality due to the transient reduction of blood supply to the bowel. In recent years, the critical contribution of gut microbiome to human health and proper gastrointestinal functions has gradually emerged. In the current study, we investigated the protective effects of five days supplementation with Bifidobacterium bifidum PRL2010 in a murine model of gut ischemia/reperfusion. Our findings indicate that animals pretreated with B. bifidum PRL2010 showed lower neutrophil recruitment in the lungs, remarkably reduced bacterial translocation and decreased transcription levels of TNFalpha and IL-10 both in liver and kidneys, at the same time increasing those of IL-12 in kidneys. Inhibiting the adhesion of pathogenic bacteria and boosting host innate immunity responses are among the possible protective mechanisms enacted by the probiotic. These results demonstrate that short-period treatment with B. bifidum PRL2010 is a potential strategy to dampen remote organ injury due to mesenteric ischemia/reperfusion.
Highlights
Mesenteric ischemia/reperfusion (I/R) is a life-threatening clinical emergency associated with different pathological conditions and surgical procedures, such as organ transplantation, bowel strangulation, vascular surgery and shock [1]
In order to assess the activity of B. bifidum PRL2010 against intestinal I/R injury under in vivo conditions, mice were pretreated with 109 CFU/day of B. bifidum PRL2010 for five consecutive days before transient occlusion of superior mesenteric artery (SMA)
Data were compared with sham operated (SO) mice supplemented with B. bifidum PRL2010 following the same experimental procedures except for SMA occlusion
Summary
Mesenteric ischemia/reperfusion (I/R) is a life-threatening clinical emergency associated with different pathological conditions and surgical procedures, such as organ transplantation, bowel strangulation, vascular surgery and shock [1]. Gut epithelial cells are highly sensitive to I/R: the poor oxygenation, which results from the transient interruption of blood supply to the bowel, followed by reperfusion triggers an intense inflammatory response that typically causes progressive distal organ impairment and, a high rate of morbidity and mortality [2]. Several studies have shown that the most important consequences of mesenteric I/R injury are the loss of intestinal mucosal barrier function and subsequent translocation of bacteria and endotoxins from the gut into the blood and to distant organs [3, 4]. Intestinal ischemia/reperfusion injury and Bifidobacterium bifidum PRL2010. Part of this research was conducted using the High Performance Computing (HPC) facility of the University of Parma
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
