Abstract
A genomic DNA library was constructed from a pathogenic strain of Aspergillus fumigatus using the cosmid vector pCosAX. Cosmid clones with homologies to the rodA, brlA, fluG, flbA or trpC genes from A. nidulans were isolated from the library. Each A. fumigatus clone was used to complement a strain of A. nidulans with a mutation in the homologous gene. A spontaneous white spored strain of A. fumigatus was isolated. The mutation was complemented by transforming the strain with a plasmid containing the wA gene from A. nidulans. The results from these experiments indicate a significant degree of structural and functional homology between genes from the organisms. These findings indicate the potential to exploit the methods and information available from A. nidulans to address questions related to human disease caused by A. fumigatus and the ability to use A. nidulans as a surrogate genetic host for characterizing A. fumigatus gene function.
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
