Abstract
The Bicolor Angelfish, Centropyge bicolor, is a tropical coral reef fish. It is named for its striking two-color body. However, a lack of high-quality genomic data means little is known about the genome of this species. Here, we present a chromosome-level C.bicolor genome constructed using Hi-C data. The assembled genome is 650Mbp in size, with a scaffold N50 value of 4.4Mbp, and a contig N50 value of 114Kbp. Protein-coding genes numbering 21,774 were annotated. Our analysis will help others to choose the most appropriate de novo genome sequencing strategy based on resources and target applications. To the best of our knowledge, this is the first chromosome-level genome for the Pomacanthidae family, which might contribute to further studies exploring coral reef fish evolution, diversity and conservation.
Highlights
Centropyge bicolor (NCBI:txid109723; FishbaseID: 5454; urn:lsid:marinespecies.org:taxname:211780) (Figure 1), known as the Bicolor, Two-Colored, or Pacific Rock Beauty Angelfish, is a showy coral reef fish commonly distributed in the Indo–Pacific ocean
A C. bicolor individual was collected from the market in Xiamen, Fujian Province, China
DNA was extracted from fresh muscle tissue according to a standard protocol
Summary
A protocols collection for BGISEQ-500, stLFR and Hi-C library construction is available in protocols.io (Figure 2) [2]. The genome size of C. bicolor was estimated as 662.27 Mbp (megabase pairs), with 37.6% repeat sequences and 1.16% heterozygous sites (Table 2, Figure 3). Protein sequences of Astatotilapia calliptera, Danio rerio, Larimichthys crocea, and Oreochromis niloticus were downloaded from the National Center for Biotechnology Information (NCBI) GenBank database and aligned to the C. bicolor genome for homolog gene annotation with Genewise (v2.4.1, RRID:SCR_015054) [22]. We firstly assembled a high-quality, chromosome-level genome of C. bicolor, with a length of 681 Mbp, and annotated 21,774 genes. This is the first genome of a fish from the Pomacanthidae family. These genomic data will be useful for genome-scale comparisons and further studies on the mechanisms underlying colorful body development and adaptation
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