Abstract
Amylin is a peptide hormone synthesized in β cells of pancreas together with insulin in response to glucose and nutrients. Amylin controls food intake and body weight but the exact mechanism is still unknown. This study aimed to examine the effects of amylin injection on hormones, metabolites and cytokines expression in rats. Wistar rats were injected amylin Intraperitoneally (IP) (10 µg kg-1 twice daily) for 7 days. Plasma and liver samples were collected for blood measurements and RT-PCR analysis. Amylin treatments induced significant decrease in body weight and food intake in time dependent manner. Moreover, amylin significantly increased insulin, leptin and lipase secretion. A decrease in plasma triglycerides, cholesterol and LPL and increase in HDL levels was recorded. There are upregulation in the expression of IL-1β and TNF-α in amylin treated rats. The results collectively indicate that amylin has anti-obesity like effects through regulation of proteins and obesity related gene expression.
Highlights
Amylin is a pancreatic 37-amino acid peptide cosecreted together with insulin from islet beta cells
This study was carried out to identify the effect of amylin on food intake and body weight
The anti-obesity like effects of amylin was clear through its effect on insulin, leptin and lipase levels
Summary
Amylin is a pancreatic 37-amino acid peptide cosecreted together with insulin from islet beta cells. Amylin secretion is stimulated by glucagon, glucagone like peptide-1and cholinergic agonists and its secretion inhibited by somatostatin and insulin (Hay et al, 2004). Functional amylin receptors are generated by coexpression of the G-protein-coupled calcitonin receptor gene and Receptor Activity-Modifying Proteins (RAMPs) (Christopoulos et al, 2003). The potential for the combination of calcitonin receptor isoforms and different RAMP proteins gives rise to at least six different subtypes of amylin receptors that display unique pharmacological properties (Hay et al, 2004). RAMP-1 and -3 mRNAs are co-localized with calcitonin receptor gene mRNA in mouse pancreatic-cells (Martinez et al, 2000). Amylin modulates the secretion and/or peripheral sensitivity of insulin, thereby regulates glucose homeostasis (Butler et al, 1990)
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