Bicarbonate Induced Redox Proteome Changes in Arabidopsis Suspension Cells.

Zepeng Yin,Sixue Chen,Craig Dufresne,Sisi Geng,Tong Zhang,Kelly Balmant,Shaojun Dai,Ning Zhu

doi:10.3389/fpls.2017.00058

Abstract

Climate change as a result of increasing atmospheric CO2 affects plant growth and productivity. CO2 is not only a carbon donor for photosynthesis but also an environmental signal that can perturb cellular redox homeostasis and lead to modifications of redox-sensitive proteins. Although redox regulation of protein functions has emerged as an important mechanism in several biological processes, protein redox modifications and how they function in plant CO2 response remain unclear. Here a new iodoTMTRAQ proteomics technology was employed to analyze changes in protein redox modifications in Arabidopsis thaliana suspension cells in response to bicarbonate (mimic of elevated CO2) in a time-course study. A total of 47 potential redox-regulated proteins were identified with functions in carbohydrate and energy metabolism, transport, ROS scavenging, cell structure modulation and protein turnover. This inventory of previously unknown redox responsive proteins in Arabidopsis bicarbonate responses lays a foundation for future research toward understanding the molecular mechanisms underlying plant CO2 responses.

Highlights

The global atmospheric concentration of carbon dioxide (CO2) has increased from 280 μmol mol−1 during the pre-industrial period to 388.5 μmol mol−1 in 2010 (NOAA/ESRL, http://www. esrl.noaa.gov/gmd/ccgg/trends/) and is projected to increase to 700 μmol mol−1 by the end of the twenty first century (Prentice et al, 2001; Aranjuelo et al, 2011)
250 μM bicarbonate was estimated to be in the cytosol of a leaf cell in ambient air (Evans and Von Caemmerer, 1996)
Previous work has shown that elevated CO2 caused Response oxygen species (ROS) production, which may lead to membrane damage (Ghezzi and Bonetto, 2003; Kolla et al, 2007; Geng et al, 2016)

Summary

Introduction

The global atmospheric concentration of carbon dioxide (CO2) has increased from 280 μmol mol−1 during the pre-industrial period to 388.5 μmol mol−1 in 2010 (NOAA/ESRL, http://www. esrl.noaa.gov/gmd/ccgg/trends/) and is projected to increase to 700 μmol mol−1 by the end of the twenty first century (Prentice et al, 2001; Aranjuelo et al, 2011). The global atmospheric concentration of carbon dioxide (CO2) has increased from 280 μmol mol−1 during the pre-industrial period to 388.5 μmol mol−1 in 2010 The influence of increasing atmospheric CO2 on plant physiology (photosynthesis, respiration, and stomatal conductance) has been studied in different species (Urban, 2003; Ainsworth and Long, 2005). A small fraction of CO2 can react with H2O to form bicarbonate (Xue et al, 2011). This interconversion between CO2 and bicarbonate can be accelerated by carbonic anhydrases (CAs), which favor the formation of bicarbonate under normal plant growth conditions (Badger, 1994).

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