Abstract
The Cu,Zn SOD catalyzes the bicarbonate-dependent oxidation of a wide range of substrates by H 2O 2. A mechanism in accord with this activity has been described. It involves the generation of a strong oxidant (Cu(I)O, Cu(II)OH, or Cu(III)) by reaction of the active site Cu with H 2O 2, followed by oxidation of bicarbonate to CO 3 − that in turn diffuses from the active site to oxidize the various substrates in free solution. Recently, an alternative mechanism, entailing firmly bound HCO 3 − and CO 3 − , has been proposed [J. Biol. Chem. 278 (2003) 21032–21039]. We present data supporting the diffusible CO 3 − and discuss the properties of this system that can be accommodated in this way and that preclude bound intermediates.
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