Abstract
Burn injuries are most challenging to manage since it causes loss of the integrity of large portions of the skin leading to major disability or even death. Over the years, hydrogels are considered as a significant delivery system for wound treatment because of several advantages over other conventional formulations. We hypothesized that the bFGF-collagen-AgSD incorporated hydrogel formulation can accelerate the rate of burn healing in animal model and would promote fibroblast cell proliferation. Neovascularization and re-epithelialization is a hall mark of burn wound healing. In the present study, histopathological investigation and scanning electron microscopy of skin tissue of Wistar rats showed almost complete epithelialisation after 16 days in the treatment group. The developed hydrogel showed significantly accelerated wound closure compared with a standard and control group. The faster wound closure resulted from increased re-epithelialization and granulation tissue formation because of the presence of collagen and growth factor. Expressions of proteins such as TrkA, p- TrkA, ERK1/2, p-ERK1/2, NF-kβ, and p-NF-kβ involved in nerve growth factor (NGF) signalling pathway were analysed by western blot. All the findings obtained from this study indicated that the hydrogel can be considered as a promising delivery system against second degree burn by faster healing.
Highlights
Burn injuries are most challenging to manage since it causes loss of the integrity of large portions of the skin leading to major disability or even death
BFGF accelerates skin regeneration through promotion of fibroblast migration and proliferation as well as it functions in the process of endothelial cell migration and proliferation promoting angiogenesis which has a potential role in the whole healing process and helps in granulation tissue formation, re-epithelialization and r emodeling[5,10,11,12]
We evaluated the expressions of some proteins such as Tropomyosin-receptor kinase A (TrkA), p-TrkA, Extracellular Regulated Kinase 1 and 2 (ERK1/2), p-ERK1/2, NF-kβ, and p-NF-kβ, involved in nerve growth factor (NGF) signaling pathway, by western blotting
Summary
Burn injuries are most challenging to manage since it causes loss of the integrity of large portions of the skin leading to major disability or even death. We hypothesized that the bFGF-collagen-AgSD incorporated hydrogel formulation can accelerate the rate of burn healing in animal model and would promote fibroblast cell proliferation. BFGF accelerates skin regeneration through promotion of fibroblast migration and proliferation as well as it functions in the process of endothelial cell migration and proliferation promoting angiogenesis which has a potential role in the whole healing process and helps in granulation tissue formation, re-epithelialization and r emodeling[5,10,11,12]. There is a possibility that proteins such as Tropomyosin-receptor kinase A (TrkA), Mitogen-activated protein kinase (MAPK) or extracellular signal-regulated kinase (ERK) involve in NGF signaling pathways may regulate the wound healing It is well-known that ERK pathway is activated by many different stimuli including growth factors and this is a major regulator of the cell. The fibroblast cell proliferation was investigated in vitro L929 mouse fibroblast cell line
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