Beziehungen zwischen proteolytischer Aktivität und Proteinabbau in höheren Pflanzen II. Der Einfluß von exogen applizierten Phytohormonen allf die proteolytische Aktivität und den Protein-Turnover in Pisum sativum

Abstract

Summary The effects of kinetin, gibberellic acid and indole acetic acid on the activities of proteinase and aminopeptidase were investigated in leaves and roots of Pisum sativum L. The influence of kinetin on protein turnover of leaves and roots of these plants was determined. Kinetin and GA 3 reduce the proteinase activity of leaves and enhance it in the roots of 14 day old plants. The effect of an IAAtreatment became visible in 28 day old plants. IAA strongly diminishes the increase of proteolytic activity which can be observed in the leaves and roots of older plants. The activity of aminopeptidase is affected only in the leaves of 14 day old plants and only by GA 3 . In kinetin-treated leaves the rate for the synthesis of protein was somewhat higher and the rate for protein degradation was somewhat diminished in comparison with the control, but the differences are not significant. In the roots kinetin stimulates protein turnover markedly. The relations between proteinase activity and gross degradation of proteins in different plant organs are discussed.