Beyond mobile phone displays: Flat panel display technology for biomedical applications

Abstract

Organ-on-Chips (OoCs) have emerged as a human-specific experimental platform for preclinical research and therapeutics testing that will reduce the cost of pre-clinical drug development, provide better physiological relevance and replace animal testing. Yet, the lack of standardization and cost-effective fabrication technologies can hamper wide-spread adoption of OoCs. In this work we validate the use of flat panel display (FPD) technology as an enabling and cost-effective technology platform for biomedical applications by demonstrating facile integration of key OoC modules like microfluidics and micro electrode arrays (MEAs) in the standardized 96-well plate format. Individual and integrated modules were tested for their biological applicability in OoCs. For microelectrode arrays we demonstrate 90–95% confluency, 3 days after cell seeding and >70% of the initial mitochondrial cell activity for microfluidic devices. Thus highlighting the biocompatibility of these modules fabricated using FPD technology. Furthermore, we provide two examples of monolithically integrated microfluidics and microelectronics, i.e. integrated electronic valves and integrated MEAs, that showcase the strength of FPD technology applied to biomedical device fabrication. Finally, the merits and opportunities provided by FPD technology are discussed through examples of advanced structures and functionalities that are unique to this enabling platform.