Abstract
Abstract The effects of 2,4-dinitrophenol (2,4-DNP) on the spectroscopic parameters (UV-Vis or FT-IR absorbance) or fluorescence emission of tryptophan and glycyl-tryptophan were studied. A quenching phenomenon of fluorescence was observed, attributed to interactions between the indole ring of the fluorophore and the aromatic ring of the quencher. The analysis of fluorescence spectra confirms that the quenching is dictated by 2,4-DNP concentration and pH. A combined mechanism of static and dynamic quenching was detected. The quenching phenomenon observed in this work could be employed to explain the mechanism of action of such compounds on large fluorescent peptides or proteins.
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