Abstract
Summary Qualitative and quantitative analysis was performed on supercritical-fluid and conventional Soxhlet extracts of Betula pendula Roth., Alnus glutinosa (L.) Gaertn., and Platanus hybrida Brot. bark. The effect of the two extraction methods on extraction yields was compared. Lupeol and β-sitosterol were identified in the bark extracts by TLC and by GC-MS. The main components were betulin and lupeol followed by β-sitosterol; betulinic acid seemed to be a minor constituent. Betulin content was determined by RP-HPLC, with acetonitrile-water 80:20 (v/v) as mobile phase. Comparison of the extraction methods showed that supercritical-fluid (scCO2 + EtOH) and ethanolic Soxhlet extraction resulted in the highest extraction yields. Accumulation of betulin derivatives was higher in supercritical-fluid extracts (scCO2 + EtOH) than in conventional Soxhlet extracts.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
