Beta-sitosterol from sablo ( Acalypha wilkesiana Muell. Arg.) leaves induce apoptosis in MCF-7 Breast cancer cell lines

Abstract

One of the most common malignancy breast cancer is highly prevalent among women. Based on the natural ingredients it contains, the plants of sablo have the potential to be employed as an anticancer therapeutic. Numerous studies have demonstrated that the chance of developing breast cancer rises with age. Additionally, the prevalence of breast cancer varies according to the different types of biased traditional Chinese medicine constitution, and the Qi-depressed constitution is highly concomitant and increases the prevalence of breast cancer. As a result, the Qi-depressed constitution is a separate risk factor for breast cancer. Previous studies have shown that the ethanol extract of Acalypha wilkesiana and its components have potential as anticancer agent against MCF-7. In the current study, using resazurin assays and flow cytometry, beta-sitosterol extracted from Acalypha wilkesiana was examined in MCF-7 human breast cancer cell lines. Resazurin experiments demonstrated its capacity to inhibit cell proliferation and trigger apoptosis, and flow cytometry was utilized to examine this capacity. Beta-sitosterol inhibits MCF-7 breast cancer cell proliferation and has an apoptotic activity that is proportional to the dose. The results of this study imply that beta-sitosterol may be used to treat cancer.