Abstract
The plant synthesizes various medicinal important compounds; similarly in vitro derived callus also synthesizes the compounds. Hence, callus can also be used as an alternative to whole plant for the production of secondary metabolites. A preliminary experiment was conducted using stem explants of Zaleya decandra (Aizoaceae) on MS, B5 and Whites media containing different concentrations of auxins and cytokinins in order to test the best suitable medium for callogenic potentiality. Murashige and Skoog (MS) medium elicited a better response than other media used. The callus formation and the nature of callus varies depending upon the nature of the growth regulators used. The various concentrations and combinations of dichlorophenoxy acetic acid (2,4-D), 6-benzylaminopurine (BAP), thidiazuron(TDZ), kinetin (Kn), naphthaleneacetic acid (NAA), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and coconut milk were tested. Of these, combination of 2,4-D (1.0 mg/L) and TDZ (2.0 mg/L) was found to be the best in inducing pigmented callus. As the concentrations of 2,4-D and TDZ increased upto 5 mgL-1, callogenic potentiality of the explants decreased. Phenotype color ranged from white/green through yellow, mezenda, violet red to red representing different types of pigments. The pigment when subjected to laboratory test using a spectrophotometer confirmed as betalains. Key words: Callus, betalain, growth regulators, medicinal plant, Zaleya decandra.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
