Beta-lactamase antimicrobial resistance in Klebsiella and Enterobacter species isolated from healthy and diarrheic dogs in Andhra Pradesh, India.

N Mohammad Sharif,D Sreenivasulu,R K Chaitanya,B Sreedevi

doi:10.14202/vetworld.2017.950-954

N Mohammad Sharif, D Sreenivasulu + Show 2 more

Open Access

https://doi.org/10.14202/vetworld.2017.950-954

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Journal: Veterinary world	Publication Date: Aug 1, 2017
Citations: 7	License type: cc-by

Affiliation: Sri Venkateswara Veterinary University

Abstract

Aim:The aim of this study was to characterize beta-lactamase antimicrobial resistance in Klebsiella and Enterobacter species isolated from healthy and diarrheic dogs in Andhra Pradesh.Materials and Methods:A total of 136 rectal swabs were collected from healthy (92) and diarrheic (44) dogs, bacteriological cultured for Klebsiella and Enterobacter growth and screened for beta-lactamase antimicrobial resistance phenotypically by disc diffusion method and genotypically by polymerase chain reaction targeting blaTEM, blaSHV, blaOXA, blaCTX-M Group 1, 2, blaAmpC, blaACC, and blaMOX genes.Results:A total of 33 Klebsiella and 29 Enterobacter isolates were recovered. Phenotypic beta-lactamase resistance was detected in 66.6% and 25% of Klebsiella and Enterobacter isolates, respectively, from healthy dogs and 66.6% and 60% of Klebsiella and Enterobacter isolates, respectively, from diarrheic dogs. Overall, incidence of extended-spectrum beta-lactamase (ESBL) phenotype was found to be 21.2% (7/33) in Klebsiella isolates, whereas none of the Enterobacter isolates exhibited ESBL phenotype. Predominant beta-lactamase genes detected in Klebsiella species include blaSHV (84.8%), followed by blaTEM (33.3%), blaCTX-M Group 1 (15.1%), and blaOXA (6.1%) gene. Predominant beta-lactamase genes detected in Enterobacter species include blaSHV (48.2%), followed by blaTEM (24.1%), blaAmpC (13.7%), and blaOXA (10.3%) gene.Conclusion:The present study highlighted alarming beta-lactamase resistance in Klebsiella and Enterobacter species of canine origin in India with due emphasis as indicators of antimicrobial resistance.

Highlights

Emergence of multidrug resistance among Enterobacteriaceae members isolated from companion animals has increased substantially over the past 20 years [1]
Phenotypic beta-lactamase resistance was detected in 66.6% and 25% of Klebsiella and Enterobacter isolates, respectively, from healthy dogs and 66.6% and 60% of Klebsiella and Enterobacter isolates, respectively, from diarrheic dogs
In a study from Wisconsin (USA), Klebsiella and Enterobacter species were isolated from 57.1% and 42.8% of healthy dog fecal samples examined, respectively [13]

Summary

Introduction

Emergence of multidrug resistance among Enterobacteriaceae members isolated from companion animals has increased substantially over the past 20 years [1]. One resistance mechanism that is of particular concern is that mediated by a family of bacterial enzymes called beta-lactamases that confer resistance to beta-lactam antibiotics [2]. Betalactamase antimicrobial resistance typically develops as a consequence of selective pressure exerted by misuse of cephalosporins [2]. Overuse and misuse of antimicrobials disrupt normal gut microbiota and select resistant bacteria, leading to enrichment of antibiotic resistant populations inside gut and emergence of so-called “superbugs” [1]. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated

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