Abstract
Glycine betaine is an important donor of methyl groups in various metabolic processes of the cell and acts as an osmoprotector when exposed to various abiotic stresses in pro- and eukaryotic organisms. Moreover, exogenous application of betaine activates the production of vitamin B12 in industrial strains-producers. In this work, we have developed a new technology for microbiological betaine synthesis that can be used in biotechnology to activate B12 biosynthesis during large-scale fermentation of Pseudomonas denitrificans.
Highlights
IntroductionVitamin B12 (cyanocobalamin): the vitamin is an antianemic factor. In the animal body, it plays a significant role in the process of hematopoiesis, the work of the red bone marrow and the biosynthesis of nucleic acids
Vitamin B12: the vitamin is an antianemic factor
At the first stage of our work, we created a chimeric gene (GSMT/DMT) consisting of fused A. halophytica glycinsarcosine methyltransferase (ApGSMT) and A. halophytica dimethylglycine methyltransferase (ApDMT) coding sequences connected by a flexible linker
Summary
Vitamin B12 (cyanocobalamin): the vitamin is an antianemic factor. In the animal body, it plays a significant role in the process of hematopoiesis, the work of the red bone marrow and the biosynthesis of nucleic acids. Known producers of cobalamin are prokaryotes and, above all, propionic and methaneforming bacteria, which form this vitamin under natural conditions. High cobalamin titers (up to 200 mg/l) were achieved for the optional anaerobe Propionibacterium shermanii, but the most common producer in the industry today is Pseudomonas denitrificans, which produces similar titers of the target product - B12 [1]. Such well-known inducers of vitamin synthesis as betaine, choline, and cobalt can make a significant contribution to optimizing the cultivation conditions of P. denitrificans as a B12 producer [2]. As one of the alternative sources of betaine, its intracellular synthesis by a recombinant B12-producing bacterium can be used, for example, due to the heterologous expression of its biosynthesis genes from available substrates
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