Beta-lactam, aminoglycoside, and quinolone resistance in Escherichia coli strains isolated from shrimps and mussels in the Marmara Sea.

Abstract

The purpose ofthe study was toexamine the prevalence ofEscherichia coli inshrimps and mussels, and todetermine the distribution ofβ-lactam, aminoglycoside, quinolone, and multi-drug resistance phenotypically andgenotypically inE.coli isolates obtained from mussels and shrimps inIstanbul. Faecal samples were collected from mussels (n=96) and shrimps (n=96) from the Marmara Sea coastline and fish markets inIstanbul. For the detection ofantibiotic susceptibilities, seven antibiotic groups were used. β-lactamase, aminoglycoside, and quinolone genes were also determined. Atotal of34 (17.7%, 15shrimps, and 19mussels) E.coli were isolated, and 17 (50%) were found tobe resistant toone ormore antimicrobials. The highest resistance was seen against aminoglycosides with 11isolates (32.35%), followed byquinolones with 10isolates (29.41%) and extended-spectrum β-lactamase (ESBL) with 4isolates (11.76%). Multi-drug resistance was detected in5isolates (14.7%) from 3shrimp and 2mussel samples. The prevalence ofESBL genes was demonstrated at3.84% inmussels and shrimp samples. There were noAmpC and carbapenemase-producing genes. These samples harbored blaCTX-M-1 (n=3) and blaTEM (n=4). Ten isolates were resistant toaminoglycosides genotypically. Resistance genes detected were strB in2isolates, aadA in5, strB and aadA together in3, ANT('')-Ia, aphA1 and aphA2 simultaneously in3, aphA1 in1, aac(3)-IIa in1isolate. aac(6')-Ib-cr gene was detected inonly one of10phenotypically resistant isolates toquinolones.