Beta‐cyclodextrin covalent organic framework coated silica composite as chiral stationary phase for high‐performance liquid chromatographic separation

Abstract

AbstractA beta‐cyclodextrin covalent organic framework coated silica composite, namely beta‐cyclodextrin covalent organic framework@SiO2, was prepared by coating the beta‐cyclodextrin covalent organic framework onto a spherical aminized silica matrix and used as the chiral stationary phase for high‐performance liquid chromatography separation in both normal‐ and reversed‐phase modes. The beta‐cyclodextrin covalent organic framework@SiO2 packed column showed good separation performance towards positional isomers such as nitrotoluene and aminophenol, and benzene homologs in the reversed‐phase high‐performance liquid chromatography mode. While chiral drug molecules including omeprazole and lansoprazole could be also enantioseparated on beta‐cyclodextrin covalent organic framework@SiO2 packed column in the normal‐phase high‐performance liquid chromatography mode. Besides, the effects of mobile phase composition, column temperature, and other factors of high‐performance liquid chromatography separation on beta‐cyclodextrin covalent organic framework@SiO2 packed column were studied in detail. Moreover, the stability test of the beta‐cyclodextrin covalent organic framework@SiO2 packed column was also investigated. The results of continuous injections and monthly detection were basically identical, suggesting the excellent durability of the obtained high‐performance liquid chromatography column. This work indicated that beta‐cyclodextrin covalent organic framework@SiO2 composite could effectively separate different compounds in high‐performance liquid chromatography normal/reversed‐phase modes, making it a promising chiral stationary phase in the field of high‐performance liquid chromatography separation.