Beta-adrenergic blockade improves uniformity of local contraction in the anterior wall of feline left ventricles.

Abstract

Uniformity of myocardial contraction has been put forward as an important regulatory mechanism of myocardial contraction, and we have previously demonstrated reduced uniformity of local myocardial contraction in the midwall of left ventricles during preload reduction combined with increased inotropy. The aim of the present study was to explore the isolated inotropic influence on uniformity of contraction, keeping loading conditions constant. Segment performance was measured by implanted piezo-electric crystals in ten open-chest, anaesthetized cats. One crystal pair, circumferential segment, aligned with midwall and epicardial fibres, whereas another perpendicular segment, longitudinal, ran close to endocardial fibre direction. Maximal systolic shortening of longitudinal segments remained unchanged following inotropic interventions, isoprenaline and timolol, whereas circumferential segments revealed reduced performance with timolol (8.4 +/- 1.0% compared with 14.0 +/- 1.3% during control; P < 0.001). In the control state the longitudinal-to-circumferential ratio (LONG/CIRC) a quantitative measure of uniformity, was 0.32 +/- 0.08. No change in uniformity occurred during isoprenaline infusion (0.32 +/- 0.11) but during beta-adrenergic blockade with timolol a clearly higher LONG/CIRC ratio was observed (0.56 +/- 0.06, P < 0.005). Myocardial tissue blood flow measurement by radioactive microspheres showed a shift in transmural distribution with interventions so that subepicardial blood flow was markedly reduced during beta-adrenergic blockade. The direction of subepicardial fibres approximates our circumferential segments. Thus, there may well exist a relationship between segmental shortening and perfusion. In conclusion, circumferential segments were most influenced by changes in inotropy in contrast to previous observations where pronounced changes in longitudinal segments with loading occur. Furthermore, this study demonstrates that reduced inotropy with timolol helped uniform local contraction as estimated by the increased LONG/CIRC ratio, a transition that could improve contraction efficacy.