Bessel beam with a micrometer-size central spot and interferometry for small volume bioliquid refractive index measurement.

Abstract

This work demonstrates an interferometric technique to measure the refractive index (RI) of microliter volume of biosample solutions with the help of an optical fiber negative axicon and Bessel beam with the micrometer-size central spot. The RI measuring device consists of a broadband superluminescent diode (SLD) source along with an optical circulator and spectrometer. The axicon optical fiber probe packaged with a glass slide is connected to a broadband SLD source through an optical circulator, and a drop of microliter bioliquid sample is placed on the packaged probe's head. The reflected light from the glass-liquid interface couples back to the axicon probe and interferes with the reference beam generated at the air-glass interface of the axicon. The interference spectrum is further analyzed by applying the fast Fourier transform to calculate power from the respective interface and solve the Fresnel equation for the RI measurement. The RI of small sample volume ∼2µl of glucose solutions and bovine serum albumin protein solutions are reported as an application of the proposed method. This sensing platform shows promising applications in biomedicine for monitoring small volume various biosample concentrations. We also demonstrate RI measurement of flowing liquid sample using the developed setup in case the design can be considered for microfluidic channel research.