Über die Virusempfindlichkeit von Schilddrüsen-Zellkulturen

Abstract

Infected tissue culture fluid of prototype strains of various viruses was passed five times in human thyroid tissue cultures. Comparative titrations were carried out with both original culture fluid and virus from the fifth cell passage in thyroid cells as well as in HeLa or monkey kidney cells respectively. By this means thyroid cells were found to exhibit an equal (or in some instances higher) susceptibility as the culture of origin to vaccinia virus, herpes simplex virus, parainfluenza viruses types 1, 2 and 3, adenoviruses types 3, 6, 9 and 12, two prototype strains of Coxsackie B3 virus, Coxsackie A9 virus, ECHO 9 virus [prototype Hill and an epidemic strain (Russ.)], an attenuated and a virulent strain of each of the three poliovirus types. The virus yield in thyroid and the original cell system was found to be in the same range. ECHO 16 virus, two prototype strains of Coxsackie B4 virus and B.V.A. 96 strain of Coxsackie B2 virus produced no cytopathic effect in thyroid cell cultures and no inapparent multiplication was demonstrable. The Ohio 1 strain of Coxsackie B2 virus had a somewhat lower infectivity for thyroid than for monkey kidney cells. Reovirus type 1 showed minimal cytopathic change and only a slight multiplication in thyroid cells. From the ECHO virus group, the prototype strains of the types 1, 2, 3, 5, 6, 7, 8, 11, 12, 13, 19, 20, 22 and 24 could be passed readily with CPE in thyroid cells, whereas ECHO types 18 and 23 were erratic and type 17 produced no CPE. The prototype strains of adenovirus types 1 to 28 as well as 24 other strains of various serotypes were cytopathic for thyroid cells without exception. Our experience with the culture and maintenance of human thyroid cells as obtained in the course of this study has been reported.