Abstract
ABSTRACT The activity of chorionic gonadotrophin (HCG) injected in solutions containing different nonspecific substances (polyvinylpyrrolidone, gelatine, tragacanth = »depot-media« is compared with the activity of HCG in distilled water. Intact immature female rats are used for the bioassays, which differ only in injection schedule or the response measured (uterine resp. ovarian weight). Depending on the injection schedule used and the response measured the results of the various bioassays in intact immature rats are different, when one and the same depot-medium is used. Significant indices of discrimination as well as significant slope differences of dose-response curves are observed. The addition of a biologically inactive kaolin extract prepared according to the »crude kaolin-acetone method« to a gonadotrophic extract from the urine of postmenopausal women produced a significant decrease in activity in one type of assay, and a significant slope difference of dose-response curves in another one. In view of these results the difficulties arising for the demonstration of qualitative differences in gonadotrophic preparations and for expressing bioassay-results in terms of the International Reference Preparation are discussed.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
