Benzothiazole Treatment Regulates the Reactive Oxygen Species Metabolism and Phenylpropanoid Pathway of Rosa roxburghii Fruit to Delay Senescence During Low Temperature Storage.

Boyu Dong,Qiuping Yao,Hongqiang Han,Xiaochun Ding,Hongmin Tang,Dequan Zhu,Kequn He

doi:10.3389/fpls.2021.753261

Boyu Dong, Qiuping Yao + Show 5 more

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https://doi.org/10.3389/fpls.2021.753261

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Abstract

Rosa roxburghii fruit were used as research objects to study the effects of different concentrations of benzothiazole (BTH) treatment on quality parameters, reactive oxygen species (ROS) metabolism, and the phenylpropanoid pathway during storage at 4°C for 14days. Results showed that BTH effectively delayed senescence with lower decay incidence, weight loss, and lipid peroxidation level and maintained the quality with higher contents of total soluble solid (TSS) content, titratable acidity (TA) in R. roxburghii fruit. Moreover, BTH increased hydrogen peroxide (H2O2) content, superoxide anion (O2•−) production rate, and the activities and expression of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), glutathione (GSH) reductase (GR), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), and peroxidase (POD), and the contents of GSH and ascorbic acid (AsA), but reduced the oxidized GSH (GSSG) content. In addition, the activities and gene expression of phenylalanine ammonia lyase (PAL), cinnamate 4-hydroxylase (C4H), and 4-coumarate-CoA ligase (4CL) and the concentrations of flavonoids, total phenols, and lignin were significantly elevated by BTH. These findings imply that BTH can delay senescence and maintain the quality of R. roxburghii fruit by modulating ROS metabolism and the phenylpropanoid pathway under low-temperature conditions.

Highlights

Rosa roxburghii (R. roxburghii), a perennial deciduous shrub from the Rosaceae family, is widely distributed in southwest China
The current study aimed to investigate the effects of BTH treatment on the following: (1) the quality including: weight loss rate, decay incidence, malondialdehyde (MDA), cell membrane permeability, titratable acidity (TA), soluble solids (TSS), and ascorbic acid (AsA) content of R. roxburghii fruit during low-temperature storage, (2) the reactive oxygen species (ROS) metabolism of R. roxburghii fruit during low-temperature storage, and (3) the phenylpropanoid pathway of R. roxburghii fruit
100 mg/L of BTH exerted the best effect on increasing AsA content compared with the 25, 50, and 200 mg/L of BTH treatments of R. roxburghii fruit

Summary

Introduction

Rosa roxburghii (R. roxburghii), a perennial deciduous shrub from the Rosaceae family, is widely distributed in southwest China. The vitamin C and superoxide dismutase (SOD) contents of R. roxburghii fruit are rich and exhibit considerable promotion prospects in the market (Liu et al, 2016; Yang et al, 2020). R. roxburghii is BTH Delays R. roxburghii Fruit Senescence prone to rot due to water loss, softening, pathogen infection, and cell wall degradation during storage at room temperature. Rotting seriously affects the appearance and quality of R. roxburghii fruit, reducing their commercial value. R. roxburghii fruit can only be stored for approximately 8 days at room temperature. Studies on the postharvest storage of R. roxburghii fruit, including those regarding 1-methylcyclopropene and bagging treatments, are relatively rare (Fan et al, 2019; Zhang et al, 2020). Reports on the effects of inducer treatment on the phenylpropanoid pathway in R. roxburghii fruit and reactive oxygen species (ROS) metabolism remain few

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