Benzothiazole tethered triazole based potential antibacterial agent as a selective fluorometric probe for the detection of Al3+ ions and phenylalanine

Abstract

The excess of Al3+ ions in the biological and environmental samples have negative impact and Phenylalanine deficiency can cause serious neurological abnormalities. Hence, there is a need of a simple and cost-effective chemosensor for the detection of Al3+ ions and Phenylalanine. Herein, we have designed a ligand that shows selectivity and sensitivity towards Al3+ ions and phenylalanine in absorption spectroscopy without any interference of other metal ions and amino acids respectively. The linearity of the B-H plot confirms the 1:1 stoichiometry ratio of the ligand with the ions and amino acid. Further, fluorescence study demonstrated that ligand shows turn-on and turn-off fluorescence in the presence of Al3+ ions and Phenylalanine with LOD values of 4.08 × 10−9 M and 18.45 × 10−6 M, respectively. The complexes of the synthesized receptor with Al3+ ions and Phenylalanine were synthesized and characterized by the 1H NMR to confirm the binding mode. Moreover, prepared molecules were subjected to in vitro microbiology assay against gram-positive and gram-negative bacteria and the results gave potential evidence of antibacterial activity against Staphylococcus Aureus and Escherichia Coli at the minimum inhibitory concentration (MIC) of 0.039 mg/ml.