Abstract

The secretion of citric acid by the bovine female genital epithelium has been investigated by Gaspar & Cseh (1963). In 60% of cases no citric acid was present, but in the secreta of 40% of the cows, quantities corresponding to spots of 1 ·5 to 40 μg could be demonstrated. In 30% of the investigated cases one unidentified spot, usually accompanied by citric acid and showing Rf values from 0·78 to 0·80, also appeared on the chromatogram. We have been trying to identify more closely the compound forming this spot. It was diffusible and remained unhydrolysed in 1 n-sulphuric acid for 2 hr and was in character acid. Although the unknown compound was generally accompanied by citric acid, it could not be identified either with lactic acid, pyruvic acid and the components of the

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