Benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester induces systemic resistance in tomato ( Lycopersicon esculentum. Mill cv. Vollendung) to Cucumber mosaic virus

Abstract

The potential of benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) to trigger systemic acquired resistance (SAR) in tomato (Lycopersicon esculentum. Mill cv. Vollendung) plants against a yellow strain of Cucumber mosaic virus (CMV-Y) was investigated. Application of BTH, as a drench, 7 days before inoculation with CMV-Y, protected plants against the necrosis caused by CMV-Y. The resistance was evident as a decreased disease incidence and severity in BTH-treated plants. Twenty-one days after challenge inoculation with CMV-Y, the disease incidence in plants with SAR did not exceed 12.5% whereas, 91.7% of control plants were severely infected. The development of primary disease symptoms in BTH-treated plants was delayed for 7 days. The disease spread rapidly in control plants and by the end of the experiment almost all control plants showed severe mosaic and leaf necrosis. Results of enzyme-linked immunosorbent assay (ELISA) indicated that BTH treatment affected virus replication in protected leaf tissues. Analysis of the newly developed leaves of BTH-treated plants for virus antigen revealed that symptomless plants failed to support the replication of CMV-Y and the concentration of the virus in these plants was similar to that in uninoculated control plants.