Benthic diatom assemblages and their correspondence with ecoregional classifications: case study of rivers in north-eastern France

Abstract

Diatoms have been routinely used to monitor the ecological status of French rivers. Since 2000, this monitoring has been carried out to comply with the Water Framework Directive. Ecoregions corresponding to the aquatic fauna and flora of rivers were originally defined on the basis of physical descriptors, designated “hydro-ecoregions” in France. Since, they have been simplified into diato-ecoregions for diatoms. In this study, data sets for benthic diatoms from rivers in north-eastern France were used to identify four main groups of diatom assemblages. The first such assemblage is found in small rivers with crystalline geologies in mountainous massifs with neutral to slightly acidic pH. The second assemblage occurs in small rivers flowing on limestone in lowland regions with an alkaline pH. The third corresponds to the assemblage found in large rivers in limestone lowland regions, and the fourth in highly mineralized rivers with high levels of chloride. Within each of these main assemblages, several communities have been characterized corresponding to different levels of pollution: from pristine to highly polluted. Multivariate analysis showed that the underlying geology was the main factor structuring the diatom assemblages, followed by the pollution level. River size had little impact on diatoms. Statistical tests showed that diato-ecoregions provided little information about diatom assemblages in the studied region, whereas there was a close correlation between diatoms and hydro-ecoregions. Nevertheless, these hydro-ecoregions did not account for the diatom assemblages in the highly mineralized rivers. These ecoregions could be further improved by merging the hydro-ecoregions of the crystalline mountainous massifs, all of which shared the same diatom assemblages.