Abstract
Cell adhesion on materials surface is critical because this phenomenon occurs before other events, as cell spreading, cell migration and cell differentiation. it is commonly accepted that the adhesion of cells on solid substrate is influenced by several substratum surface properties, such as wettability, surface charge, roughness and topography. plasma technique is a convenient method for modifying surface properties of materials without affecting physical properties. in this study, poly(lactide-co-glycolide), plga, membranes were modified by oxygen and nitrogen plasma to improve polymer hydrophilicity and verify their effect on vero cells culture. the plga membranes, which were characterized by sem and contact angle, showed increased surface rugosity and narrower contact angles. cell adhesion, cytotoxicity assay, sem and cytochemistry analysis showed that plasma treatment was beneficial to cell growth by improving cell-polymer interaction. Cell adhesion on materials surface is critical because this phenomenon occurs before other events, as cell spreading, cell migration and cell differentiation. It is commonly accepted that the adhesion of cells on solid substrate is influenced by several substratum surface properties, such as wettability, surface charge, roughness and topography. Plasma technique is a convenient method for modifying surface properties of materials without affecting physical properties. In this study, poly(lactide-co-glycolide), PLGA, membranes were modified by oxygen and nitrogen plasma to improve polymer hydrophilicity and verify their effect on Vero cells culture. The PLGA membranes, which were characterized by SEM and contact angle, showed increased surface rugosity and narrower contact angles. Cell adhesion, cytotoxicity assay, SEM and cytochemistry analysis showed that plasma treatment was beneficial to cell growth by improving cell-polymer interaction.
Highlights
A research area that has received increasing attention is tissue engineering that restores tissue functionality from native or synthetic sources by applying engineering principles
Whenever biomaterials are employed it is essential to understand their pattern of cell growth and differentiation and select the appropriate polymer, given that the latter may influence the development of bioresorbable polymers designed to stimulate the regeneration of damaged tissues[2]
The results showed an increase of hydrophilicity of PLGA membranes
Summary
A research area that has received increasing attention is tissue engineering that restores tissue functionality from native or synthetic sources by applying engineering principles. Whenever biomaterials are employed it is essential to understand their pattern of cell growth and differentiation and select the appropriate polymer, given that the latter may influence the development of bioresorbable polymers designed to stimulate the regeneration of damaged tissues[2]. The importance of cell culture methodology related to biomaterials research is that it enables fast evaluation of the performance of polymers. Evaluation of cell-biomaterial interactions concerning the prediction of possible in vivo reactions to these polymers is invaluable when they are utilized as substitutes for body parts or as a stimulant for regeneration or repair of damaged tissues[1]. Cell adhesion is closely related to surface properties of biomaterials. It is generally accepted that adhesion of cells onto solid substrate is influenced by several substratum surface properties, such as wettability[4], Biodegradable polyester, including poly(lactide-coglycolide) (PLGA) with various lactide/glycolide ratios, is an important material for tissue engineering[8], despite its poor hydrophilicity and lack of natural cell recognition sites on its surface[9]
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