Beneficial Role of Nigella Sativa Extracted Oil on the Hepato-toxic Effect of Ceftriaxone in Adult Male Albino Rat (Histological and Immuno-histochemical Study)

Abstract

Introduction: Ceftriaxone is a broad-spectrum cephalosporin with a hepatotoxic effect. Nigella sativa (Ns) has potentantioxidant effects, so it has a promising medicinal application.Aim: To study the possible protective role of Nigella Sativa Extracted Oil against the Hepato-toxic Effect of Ceftriaxone inadult male albino rat.Materials and Methods: Thirty two adult male albino rats had been divided into four groups with 8 rats allocated in eachgroup: Group 1 (control group), Group 2 (Ns oil administrated): received Nigella sativa (NS) oil 2 ml/kg by gastric tube dailyfor 4 weeks, Group 3 (ceftriaxone treated): received ceftriaxone (360 mg/kg) (I.P) daily for 4 weeks and Group 4 (Ns oil andceftriaxone treated group). At the last day of the experiment, the liver specimens of each animal were dissected carefully andwere processed for light-microscopic examination.Results: Ceftriaxone treated group revealed elevated level of liver enzymes that were relatively lower in ceftriaxone andNs oil group. Histological examination of the liver of ceftriaxone treated group showed dilated congested central vein andsinusoids with hydropic degeneration of hepatocytes, while portal triad revealed dilated congested portal vein and dilated bileducts surrounded by inflammatory cells. Additionally, abundant deposition of collagen was detected and increased expressionof TNFα immune staining within hepatocytes and in lining of sinusoids. All these histological alterations were less marked ingroup (4) which received NS oil in combination with ceftriaxone.Conclusion: Ns oil has a protective effect against liver injury resulted from ceftriaxone injection due to its antioxidant activities.