Abstract
Short dry spells are an important grain yield constraint in tropical regions. Plant growth-promoting bacteria (PGPB) and their metabolites can mitigate the impact of drought stress by promoting changes in plant metabolism, physiology, and biochemistry. However, the effects of PGPB on soybean [Glycine max (L.) Merril] under drought stress in tropical regions have not been established. The experiments were carried out under tropical field conditions with short dry spells. Therefore, in this study we used a three-factorial trial to evaluate the effects of bacterial consortium consisting of N2-fixing Bradyrhizobium japonicum (strain SEMIA 5079) and Bradyrhizobium diazoefficiens (strain SEMIA 5080), the biocontrol agent Bacillus subtilis (strain QST 713), and the plant growth-promoting Azospirillum brasilense (strains Ab-V5 and Ab-V6) with or without application of microbial secondary metabolites (MSM, rhizobial metabolites enriched in lipo-chitooligosaccharides (LCOs)) during two growing seasons. Photosynthetic pigments, gas exchange parameters, antioxidant enzyme activity and proline concentrations in leaves, nodulation, plant growth development and grain yield were evaluated. The bacterial consortium comprising Bradyrhizobium spp., A. brasilense strains and MSM application increased the contents of chlorophyll a (14.5 %), chlorophyll b (30.8 %), total chlorophyll (17.2 %), and total carotenoids (27.3 %) compared with Bradyrhizobium spp. treatment alone. This consortium also increased the net photosynthetic rate (17.7 %), stomatal conductance (56.5 %), internal CO2 concentration in the substomatal chamber (8.3 %), and transpiration (44 %) compared with plants that received the standard inoculation (Bradyrhizobium spp. only), while reducing the leaf contents of hydrogen peroxide (−18.8 %) and proline (−29.4 %), lipid peroxidation (−15.9 %), and the activities of superoxide dismutase (−18.2 %), catalase (−21.2 %), and ascorbate peroxidase (−19.1 %). Taken together, the results indicate that a beneficial bacterial consortium comprising Bradyrhizobium spp. and A. brasilense strains combined with MSM application can alleviate oxidative damage during dry spells. Furthermore, this consortium improved soybean nodulation, plant growth development, and grain yield by up to 12.2 %.
Highlights
Adverse environmental factors are increasingly impacting agriculture worldwide (FAO, 2019)
To guarantee global food security, mechanisms to mitigate the effects of abiotic stresses caused by climate change have been investigated in several crop species
Plants inoculated with A. brasilense strains exhibited higher concentrations of these pigments compared to plants that were not inoculated with A. brasilense, and application of microbial secondary metabolites (MSM) further increased these levels
Summary
Adverse environmental factors are increasingly impacting agriculture worldwide (FAO, 2019). Due to its high capacity for biological nitrogen fixation (BNF), soybean plays a key role in agriculture sustainability (Hungria and Mendes, 2015; Ciampitti and Salvagiotti, 2018; Moretti et al, 2018). Variations in the climate characteristics of tropical regions used for cropping (Alvares et al, 2013; Cunningham, 2020) result in differences in the intensity, frequency, and timing of water supply (Hu and Wiatrak, 2012; Battisti and Sentelhas, 2014). Water deficit is a major limiting factor of soybean yield and can be mitigated at least partially by using cultivars with appropriate physiological traits and crop management (Battisti and Sentelhas, 2017)
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